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KMID : 0545120070170020271
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Journal of Microbiology and Biotechnology
2007 Volume.17 No. 2 p.271 ~ p.279
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Black Rice (Oryza sativa L. var. japonica) Hydrolyzed Peptides Induce Expression of Hyaluronan Synthase 2 Gene in HaCaT Keratinocytes
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Sim Gwan-Sub
Lee Dong-Hwan
Kim Jin-Hwa
An Sung-Kwan
Pyo Hyeong-Bae
Lee Bum-Chun
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Abstract
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Black rice (Oryza sativa L. var. japonica) has been used in folk medicine in Asia. To understand the effects of black rice hydrolyzed peptides (BRP) from germinated black rice, we assessed the expression levels of about 20,000 transcripts in BRP-treated HaCaT keratinocytes using human 1A oligo microarray analysis. As a result, the BRP treatment showed a differential expression ratio of more than 2-fold: 745 were activated and 1,011 were repressed. One of the most interesting findings was a 2-fold increase in hyaluronan synthase 2 (HAS2) gene expression by BRP. Semiquantitative RT-PCR showed that BRP increased HAS2 mRNA in dose-dependent manners. ELISA showed that BRP effectively increased hyaluronan (HA) production in HaCaT keratinocytes
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KEYWORD
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Oryza sativa, germinated black rice, microarray, peptides, hyaluronan synthase
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